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Distribution of Candida isolates by a source of isolation.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Distribution of Candida isolates by a source of isolation.

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques: Isolation

Species distribution of 110 clinical isolates. The 110 Candida strains analyzed included 40 C . albicans (36.36%), 37 C . parapsilosis (33.64%), 21 C . tropicalis (19.09%), 9 C . glabrata (8.18%), 2 C. rugose (1.82%), and 1 C . haemulonii (0.91%) isolate.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Species distribution of 110 clinical isolates. The 110 Candida strains analyzed included 40 C . albicans (36.36%), 37 C . parapsilosis (33.64%), 21 C . tropicalis (19.09%), 9 C . glabrata (8.18%), 2 C. rugose (1.82%), and 1 C . haemulonii (0.91%) isolate.

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques:

Comparison of the virulence factors among Candida strains isolated from different sources (mean optical density ± SD).

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Comparison of the virulence factors among Candida strains isolated from different sources (mean optical density ± SD).

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques: Comparison, Isolation

Dendrogram presenting the genetic relatedness of 107 Candida spp. based on the random amplified polymorphic DNA (RAPD) data. The virulence factors (i.e., caseinase, hemolysin, and biofilm), drug resistance, and specimen sources are indicated in the figure. The vertical line divides the strains based on the level of genetic similarity into related and unrelated strains. The red boxes indicate azole resistance (fluconazole and voriconazole), whereas the blue boxes indicate azole-susceptible (S) dose dependence (SDD). Red ellipses represent resistance to echinocandins, while the blue ellipses represent intermediate resistance to micafungin. (A) Dendrogram of 40 C. albicans isolates. (B) Dendrogram of 37 C. parapsilosis isolates. (C) Dendrogram of 21 C. tropicalis isolates. (D) Dendrogram of 9 C. glabrata isolates.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Dendrogram presenting the genetic relatedness of 107 Candida spp. based on the random amplified polymorphic DNA (RAPD) data. The virulence factors (i.e., caseinase, hemolysin, and biofilm), drug resistance, and specimen sources are indicated in the figure. The vertical line divides the strains based on the level of genetic similarity into related and unrelated strains. The red boxes indicate azole resistance (fluconazole and voriconazole), whereas the blue boxes indicate azole-susceptible (S) dose dependence (SDD). Red ellipses represent resistance to echinocandins, while the blue ellipses represent intermediate resistance to micafungin. (A) Dendrogram of 40 C. albicans isolates. (B) Dendrogram of 37 C. parapsilosis isolates. (C) Dendrogram of 21 C. tropicalis isolates. (D) Dendrogram of 9 C. glabrata isolates.

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques: Amplification

Minimum inhibitory concentration values (μg/mL) for the Candida isolates.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Minimum inhibitory concentration values (μg/mL) for the Candida isolates.

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques: Concentration Assay

Virulence level (biofilm biomass, caseinase activity, and hemolysin activity) of the 107 Candida species isolates from different specimen sources. Y-axis (far left panel): biofilm biomass (positively correlated with virulence); Y-axis (far right panel): the Pz values of caseinase and hemolysin activities (negatively correlated with virulence). No significant differences were noted in these factors. (A) The virulence level of 40 C. albicans isolates from different specimen sources. (B) The virulence level of 37 C. parapsilosis isolates from different specimen sources. (C) The virulence level of 21 C. tropicalis isolates from different specimen sources. (D) The virulence level of 9 C. glabrata isolates from different specimen sources.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Candida Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital

doi: 10.3389/fcimb.2021.721439

Figure Lengend Snippet: Virulence level (biofilm biomass, caseinase activity, and hemolysin activity) of the 107 Candida species isolates from different specimen sources. Y-axis (far left panel): biofilm biomass (positively correlated with virulence); Y-axis (far right panel): the Pz values of caseinase and hemolysin activities (negatively correlated with virulence). No significant differences were noted in these factors. (A) The virulence level of 40 C. albicans isolates from different specimen sources. (B) The virulence level of 37 C. parapsilosis isolates from different specimen sources. (C) The virulence level of 21 C. tropicalis isolates from different specimen sources. (D) The virulence level of 9 C. glabrata isolates from different specimen sources.

Article Snippet: The FKS1 sequences were compared with the reference sequences of C. tropicalis ATCC 750 (Gene ID: EU676168) and C. glabrata (Gene ID: XM_446406).

Techniques: Activity Assay